DNA insert from MON-Ø4Ø32-6

The transforming plasmid PV-GMGT04 contained a transfer DNA (T-DNA) region comprising an expression cassette encoding the glyphosate tolerance protein CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS), derived from Agrobacterium tumefaciens strain CP4. Transformation of soybean was achieved through particle bombardment.
-Transcription of the cp4 epspscoding sequence is directed by an enhanced 35S promoter from Cauliflower mosaic virus. The coding region includes the chloroplast transit peptide CTP4 derived from Petunia hybrida, which directs the expressed protein to the chloroplast. Transcription is terminated by the nopaline synthase (NOS) terminator from Agrobacterium tumefaciens. Expression of this cassette results in production of the CP4 EPSPS protein, which has reduced affinity for glyphosate and allows continued synthesis of aromatic amino acids through the shikimate pathway in the presence of the herbicide.

Note:
Molecular characterization demonstrated that a single functional copy of the intended transformation cassette containing the cp4 epsps coding sequence was integrated into the soybean genome. Analysis indicated that no vector backbone DNA was integrated.

DNA insert from ACS-GMØØ6-4

The transforming plasmid pB2/35SAcK (also designated pWRG5143) contained an expression cassette encoding phosphinothricin N-acetyltransferase (PAT), derived from Streptomyces viridochromogenes. Transformation of soybean was achieved through particle bombardment.
-Transcription of the pat coding sequence is directed by the 35S promoter from Cauliflower mosaic virus. Transcription is terminated by the 35S terminator from Cauliflower mosaic virus. Expression of this cassette results in production of the PAT protein, which inactivates glufosinate through acetylation, thereby preventing inhibition of glutamine synthetase and allowing normal plant growth following herbicide application.

Note:
Molecular characterization demonstrated that a single copy of the pat expression cassette was integrated into the soybean genome. Partial fragments of the bla gene were also detected flanking the pat cassette; however, these fragments are not expressed in the host organism.

For additional information on the molecular characterization of these inserts, kindly refer to the parental LMO records.